5-Carboxytetramethylrhodamin - 5-Carboxytetramethylrhodamin

Structural formula
Structure of 5-carboxytetramethylrhodamine
Name 5-Carboxytetramethylrhodamin
other names


Sum formula C 25 H 22 N 2 O 5
External identifiers / databases
CAS number 91809-66-4
PubChem 2762602
ChemSpider 2043318
Wikidata Q27122704
Molar mass 430.45 g · mol −1 [1]
safety instructions
GHS hazard labeling [1]
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases [1]
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

5-carboxytetramethylrhodamine ( 5-TAMRA ) is a red fluorescent dye from the group of rhodamines . It can be classified both in the group of triphenylmethane dyes and xanthene dyes . 5 ‑ TAMRA is used for fluorescent labeling of nucleic acids and proteins . [2] [3] The carboxy group can be converted into a reactive intermediate state with coupling reagents . Alternatively, the succinimidyl ester can be 5-carboxytetramethylrhodamine- N- succinimidyl ester( 5-TAMRA-SE ) can be used for marking. 5-TAMRA is used, among other things, for fluorescent substrates of enzymes , hybridization probes and in FRET .

Individual evidence

  1. a b c Data sheet 5-Carboxytetramethylrhodamine from Sigma-Aldrich , accessed on October 31, 2016 ( PDF ).
  2. Maksim V. Kvach, Dmitry A. Tsybulsky, Vadim V. Shmanai, Igor A. Prokhorenko, Irina A. Stepanova, Vladimir A. Korshun: Current Protocols in Nucleic Acid Chemistry. John Wiley & Sons, Inc., 2001, ISBN 978-0-471-14270-6, Kapitel Non-Nucleoside Phosphoramidites of Xanthene Dyes (FAM, JOE, and TAMRA) for Oligonucleotide Labeling, doi:10.1002/0471142700.nc0455s52, PMID 23512693.
  3. G. H. Lee, E. J. Lee, S. S. Hah: TAMRA- and Cy5-labeled probe for efficient kinetic characterization of caspase-3. In: Analytical biochemistry. Band 446, Februar 2014, S. 22–24; doi:10.1016/j.ab.2013.10.013, PMID 24144487.